5 Progesterone Protocols, Synchronization of Ovulation and Summary

Transcript

Greetings friends!

As I told you in the last class, today we are continuing with CIDR and PGF2 Alpha and various combinations and it will be interesting to watch.

This slide shows the CIDR/ TRIU-B +PGF2 Alpha combination for the Synchronisation of Estrous. You consider today’s (day 0) animal Estrous. Either day 10 means that it is in Diestrus or you can use it on day 6. From day 6 onwards day 10 you are inserting 8 days you keep the CIDR. On the 18th day you give PGF2 Alpha.19th day you remove the CIDR. 21st day and 22nd day you do AI (Artificial Insemination). Once again, I will repeat. Day 0 is Estrous day, day 10 insert CIDR/TRIU-B, day 18 give PGF2 Alpha, day 19 remove the CIDR, day 21 and 22 do AI. This is the best protocol for the Synchronisation of Estrous. Suppose if the animal is anestrous, insert the CIDR on day 0 then 8th day instead PGF2 Alpha, give GnRH 5ml (20 micrograms) Intramuscularly or eCG about 500 IU to 1000 IU you give Intramuscularly. 9th day you remove CIDR and day 11 and 12 do AI. At the time of AI again you can give GnRH, 2.5 ml Intramuscularly. So, this is for two hornist animals, both the ovaries are Smooth. Previously I told day 10 CIDR, day 18 PGF2 Alpha, day 19 removal of CIDR, day 21 and 22nd doing AI. It is in the cyclical animals. If it is in an anestrous animal, this protocol is found to be the best protocol.So a combination of CIDR plus GnRH or eCG. It is not PGF2 Alpha, it is GnRH /PGF2 Alpha in true anestrous animals.

OK, the next protocol we are using is a Synchronisation of Ovulation. This is called synchronisation of Ovulation. So, whatever we used so far, it is Synchronisation of Estrous.

So the next step in this synchronisation program is the Synchronisation of Ovulation. Precisely it will synchronise the ovulation so that the consumption rate will be increased. The first protocol in the synchronisation of Ovulation is the Ovsynch protocol.

Suppose GnRH is given on day 0 and day 7 you give PGF2 Alpha, then on day 9 after 48 hours give GnRH again. After the 2nd GnRH injection, 16 to 18 hours later go for AI. This is called GPG protocol. Suppose an animal is cyclical, day 0 is Estrous, day 6 or7 you start this program. Suppose on day 6 you are starting the GnRH then 7 days later,i.e., on day 13, you will give PGF2 Alpha. Then two days later, on day 15, you will be going for GnRH injection, then on day 16, you will do AI. So, this protocol is the best protocol for the precise synchronization of Ovulation. This is called GPG protocol. Here the GnRH and the PGF2 Alpha are used. This (PPT) is very well explaining the Ovsynch protocol, that is GnRH, where Receptacle or Fertagyl is used to ovulate the dominant follicle. It may be present on day 6 or day 5, so you can give GnRH day 6 then 7 days later give PGF2 Alpha, then 48 hours later, i.e., after the PGF2 Alpha give GnRH, then 16 to 18 hours later, go for a Fixed Time AI.So this (G) is for synchronization of Estrous, this (P) protocol is for 16 to 24 hours before AI, this (G)we are giving GnRH to synchronize Ovulation also. So this is called a GPG Protocol or Ovsynch Protocol.

The scientists have improved the Ovsynch Protocol. So, if you don’t know which day the animal expresses the Estrous, 12 days before the GnRH injection, give PGF2 Alpha to the cyclical animal. So, animals will come to heat within 3 days, and it will be on day 6 or 7 again start the GnRH Protocol, GPG protocol i.e., GnRH- PGF2 Alpha-GnRH. This is called Presynch- Ovsynch Protocol. Then 16 to 18 hours later go for AI, this is called Presynch-Ovsynch Protocol.

Next is Pre synchronization: Presynch Ovsynch can be done with two PGF2 Alpha. First give PGF2 Alpha then 14 days later you give PGF2 Alpha, again 14 days later start the GPG protocol i.e., (GnRH- PGF2 Alpha-GnRH), then go for AI. So, if you see this protocol, you can follow any time. So this is very important and it is giving more chances for consumption in animals.

Next, we will see a G6G program.

Actually, in the Presynch Ovsynch Protocol, we give PGF2 Alpha then 14 days later we will give PGF2 Alpha again and 14 days or 12 to 14 days later we will start the Synchronisation Ovulation program. But in the G6G program, we will give PGF2 Alpha,2 days later we will give GnRH, 6 days later we will again give GnRH.This is the first GnRH of the Ovsynch program. Then we will continue the Ovsynch protocol. This proved that it is giving more consumption rate than any other protocol.

Next, the Double Ovsynch protocol is also recommended. That is double the time you are doing synchronization of the Ovulation program. Give GnRH, 7 days later give PGF2 Alpha, again 3 days later give 2nd GnRH, then 7 days later you start the 3rd GnRH, and you are continuing the Ovsynch protocol. This is called a Double Ovsynch. So, there are many protocols available, and I have told you, whichever protocol you prefer in the field according to the availability of the drug in your area, you can choose. Many avenues are available in reproduction to improve the fertility rate in infertile animals.

Now, look at this Cosynch Programme. In GnRH, we have to visit the GPG protocol many times. But in the Cosynch Programme, there is a difference. The difference between Ovsynch and Cosynch is that 2 days later, we will give a PGF2 Alpha,48 hours after the PGF2 Alpha injection. But hereafter the PGF2 Alpha injection, at the time of AI we will go for GnRH injection. So, 16 to 18 hours before the AI we will give a GnRH injection. But here we are not doing that, you can have these at day 10, So on day 10 along with AI, you can go for GnRH injection also. So, one more visit you are avoiding in Cosynch protocol. so, at the time of AI itself, you are administering the GnRH. This is called a Cosynch Programme.

Next is the Heatsink Programme. Suppose if you don’t have GnRH. This is Heatsynch Protocol, day 0 you are giving GnRH, day 7 PGF2 Alpha, day 8 giving E2 (Esterol Benzoate) or Caproate. So Esterol Benzoate any Estrogen can be given, then day 10 we are doing Timed Artificial Insemination(TAI). So, this is called Heatsynch Protocol.

The next protocol is the CIDR protocol. So Ovsynch + CIDR you can combine together.GnRH insert CIDR, 7th day give PGF2 Alpha and remove CIDR, 9th day give GnRH and 10th day you can go for Breeding. In Cosynch + CIDR also give GnRH insert CIDR,7th day give PGF2 Alpha, then 10th day or after the 72 hours of PGF2 Alpha injection, give GnRH and Breed the animal. So, this is Cosynch +CIDR.

In Select D Synch + CIDR, give GnRH insert CIDR, remove the CIDR on day 7, breed the animal by deducting the estrous. This is called Select D Synch. So, Select D Synch means to give GnRH, insert CIDR, 7th day give PGF2 Alpha, remove the CIDR, breed the animal by detecting the Estrous. So, there are many protocols available. All these protocols can be used to treat Repeat breeders, Anestrus and Cystic vary. So, in Cystic Ovary also this can be used. So, by way of using the Estrous Synchronisation Programme, many infertility cases can be treated.

I think all of you will utilize this program for improving the fertility of infertile cows and buffaloes under field conditions.

What all are the points to be remembered in the Estrous Synchronisation program:

• Observe the cyclicity pattern of the animal.
• Select the appropriate protocol according to the owner’s status, ability, patience, and everything. You select the best protocol for cyclical and Anestrous animals. Best is Prostaglandin for cyclical animals and Progesterone for cyclical or non-cyclical animals.
• Ensure ovulation by adding GnRH in the selected protocols
• Recommended performing AI at 24 hours intervals in Estrous Synchronisation programs.
• So, you can do double AI in any synchronization program.

These are the points to be remembered.

Dear friends, so in the entire series of classes we have seen the Estrous synchronization technique in cows and buffaloes, which is an important technique to augment fertility both in normal cycling and the infertile animals, so you can use this technique for improving fertility under field condition to increase the economy of the poor farming community of rural India.

And at anytime and anyplace from anywhere, you can contact me at this address.

Thanks to one and all.